Thermo Fisher Scientific CXCL12 beta (SDF-1 beta) Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

-

Immunoprecipitation (IP)

1:300-1:500

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468512

Product Specific Information

Description: The polyclonal antibody reacts with both mouse and rat CXCL12 beta (SDF-1 beta). It was generated using E. coli-expressed N terminal amino acids 20-93 of mouse CXCL12 beta as an immunogen. CXCL12 beta, also known as pre-B-cell growth-stimulating factor (PBSF) and stromal cell-derived factor-beta (SDF-1 beta), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of CXCL12 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice. CXCL12 has been shown to be chemotactic for lymphocytes. In addition, CXCL12 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. CXCL12 binding to CXCR4 inhibits HIV-1 entry.

Applications Reported: This polyclonal can be used in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CXCL12 beta of other species has not been determined.

Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows:

Western Blot 1:1000

Immunoprecipitation 1:300-500

Immunohistochemistry 1:100-500.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.