Thermo Fisher Scientific 5-Methylcytosine Recombinant Rabbit Monoclonal Antibody (2G2M3)

Applications

Tested Dilution

Publications

ELISA (ELISA)

1 µg/mL

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Peptide Array (Array)

1:500-1:1,000

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Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2G2M3

Immunogen

Chemical compounds corresponding to m5C.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.14 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.