Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr493, Tyr526) Recombinant Rabbit Monoclonal Antibody (Zap70Y493-H11)
Recombinant rabbit monoclonal antibody targeting phospho-ZAP70/Syk (Tyr493, Tyr526). High specificity and sensitivity for WB and Flow Cytometry. Consistent performance with animal origin-free formulation. Suitable for T-cell signaling research.
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Applications
Western Blot (WB)
- Assay-dependent
Flow Cytometry (Flow)
- 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Zap70Y493-H11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2745064 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B between SH2 and kinase domains.
- Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhance ZAP70 function in lymphocyte signaling.
- Tyr292 phosphorylation terminates transient activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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