
Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (TLD-3A12), Biotin, eBioscience
쥐 CD31(PECAM-1)을 인식하는 단클론 항체로, Biotin으로 표지되어 있습니다. 유세포 분석(Flow Cytometry)에 적합하며, 고순도의 액상 형태로 제공됩니다. 0.5 mg/mL 농도로 PBS(pH 7.2)에 보관되며, 4°C 암소 보관을 권장합니다.
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Applications
- Flow Cytometry (Flow)
Tested dilution: 0.25 µg/test
View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLD-3A12 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572763 |
Additional Formats
Product Specific Information
The TLD-3A12 monoclonal antibody reacts with rat CD31 (platelet-endothelial cell adhesion molecule-1, PECAM-1, gpIIa). This 130-kDa surface protein is highly expressed on endothelial cells and at low levels on leukocytes and platelets. CD38 binds to CD31, and homotypic CD31 interactions are important in adhesion, cell-cell and cell-matrix interactions, and signal transduction.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on rat splenocytes at ≤0.25 µg/test. One test is defined as the amount of antibody to stain a cell sample in 100 µL final volume. Optimal titration is recommended for best performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular system, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immunoregulation, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis, and thrombosis, making it a key regulator in vascular biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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