Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), Biotin, eBioscience
인간 CD34 항원 인식용 단클론 항체로, Biotin 결합형입니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 혈액세포 및 줄기세포 표지 연구에 적합하며, 4°C 암소 보관 권장.
- 카탈로그번호
- 13-0349-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), Biotin, eBioscience
Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 48 publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H11 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10668008 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 is a single-pass transmembrane protein with a heavily glycosylated extracellular and N-terminal mucin domain. It is expressed on hematopoietic progenitors, mesenchymal stem cells, tumor cell lines, and vascular endothelia in adults.
Epitopes of CD34 have been classified into three classes (I, II, III) based on enzymatic cleavage sensitivity. The 4H11 antibody belongs to class III, indicating reactivity with a protein epitope.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of normal human peripheral blood cells (≤0.5 µg/test, in 100 µL final volume). Cell count should be empirically determined (10⁵–10⁸ cells/test). Titrate for optimal performance.
Target Information
CD34 is a highly glycosylated surface protein (111–115 kDa) expressed on stem cell populations. It serves as a stem cell marker and may function in receptor-mediated endocytosis, adhesion, differentiation, and proliferation of hematopoietic stem cells. CD34 expression reflects specific stages of hematopoietic development and may mediate cell attachment to the bone marrow matrix or stromal cells.
CD34 possibly acts as a scaffold for lineage-specific glycans, facilitating interactions with lectins. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction.
Diseases associated with CD34 include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.