Thermo Fisher Scientific CD28 Monoclonal Antibody (37.51), Biotin, eBioscience

Thermo Fisher Scientific CD28 Monoclonal Antibody (37.51), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells, and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2), acting as a potent costimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases the cytotoxicity of CD3-activated T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.5 µg per test (100 µL final volume). Cell number may vary from 10^5 to 10^8 cells/test. Antibody titration is recommended for optimal performance.

Note: Extended incubation (30–45 minutes) can improve staining results.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T-cell surface glycoprotein of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and serves as a critical costimulatory receptor by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering an anergic state or undergoing premature apoptosis. In murine lymphoid organs, all CD4+ and CD8+ T cells express CD28, with varying expression levels depending on maturation stage. Diseases associated with CD28 dysfunction include mycosis fungoides and Sezary's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.