Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Biotin, eBioscience
인간 CD28 항원을 인식하는 Biotin 결합 단클론 항체로, T세포 활성화 및 Flow Cytometry 분석에 적합합니다. 0.5 mg/mL 농도의 액상 시약이며, PBS buffer와 sodium azide를 포함합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Biotin, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 1 µg/test | View 21 publications |
| Functional Assay (Functional) | - | View 4 publications |
| T-Cell Activation (TCA) | - | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD28.2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466415 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 660
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 binds CD80 (B7-1) and CD86 (B7-2) and serves as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg/test (100 µL final volume, cell number empirically determined between 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell surface glycoprotein belonging to the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical costimulatory receptor providing the second activation signal via binding to CD80 and CD86 on antigen-presenting cells.
CD28 prevents T cells from entering an anergic state or undergoing premature apoptosis. In murine lymphoid organs, CD28 is expressed on all CD4⁺ and CD8⁺ T cells. Expression is highest on immature thymocytes and lower on mature cells.
Diseases associated with CD28 dysfunction include mycosis fungoides and Sezary’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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