Thermo Fisher Scientific EBP50 Polyclonal Antibody
Rabbit polyclonal antibody targeting human EBP50 (NHERF1), validated for Western blot at 1:1,000 dilution. Unconjugated, liquid form purified by antigen affinity chromatography. Suitable for research use only; stores at -20°C.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide surrounding Ala140 of human NHERF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 8 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11007644 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions. It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM-binding region and two N-terminal PDZ domains, enabling head-to-tail intramolecular conformation to regulate interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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