Thermo Fisher Scientific Phospho-c-Jun (Thr239) Polyclonal Antibody
Phospho-c-Jun (Thr239) 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC(P), IP에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 1 mg/mL 액상 형태로 제공되며, 4°C 또는 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Threonine 239 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553625 |
Product Specific Information
- Detects endogenous protein at molecular weights of 43 and 48 kDa.
- Purity >95% by SDS-PAGE.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
It promotes NR5A1 activity when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP pathway stimulation.
Also involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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