Thermo Fisher Scientific Phospho-c-Jun (Tyr170) Polyclonal Antibody
Phospho-c-Jun (Tyr170) 특이적 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응. Western blot 및 IHC(파라핀) 실험에 최적화. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로 4°C 또는 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- Publications: [참조 없음]
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
- Publications: [참조 없음]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Tyrosine 170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553626 |
Product Specific Information
- Detects endogenous protein at molecular weights of 43 and 48 kDa.
- Purity >95% by SDS-PAGE.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. It is involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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