Thermo Fisher Scientific Caldesmon Polyclonal Antibody
Rabbit polyclonal antibody targeting human Caldesmon (CALD1). Validated for WB and ELISA. Recognizes cytoplasmic and cytoskeletal Caldesmon isoforms. Supplied as liquid form, affinity purified, in PBS with 50% glycerol. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–240 of human CALD1 (NP_004333.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806490 |
Product Specific Information
Immunogen sequence:
MDDFERRREL RRQKREEMRL EAERIAYQRN DDDEEEAARE RRRRARQERL RQKQEEESLG QVTDQVEVNA QNSVPDEEAK TTTTNTQVEG DDEAAFLERL ARREERRQKR LQEALERQKE FDPTITDASL SLPSRRMQND TAENETTEKE EKSESRQERY EIEETETVTK SYQKNDWRDA EENKKEDKEK EEEEEEKPKR GSIGENQIKD EKIKKDKEPK EEVKSFMDRK KGFTEVKSQN
Positive Samples: HeLa, 22Rv1, U-251MG, U-87MG
Cellular Location: Cytoplasm, cytoskeleton, myofibril
Target Information
Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified, differing in electrophoretic mobility and cellular distribution.
- h-caldesmon (120–150 kDa): Predominantly expressed in smooth muscle
- l-caldesmon (70–80 kDa): Found in non-muscle tissues and cells
Neither variant is detected in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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