Thermo Fisher Scientific RFP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560939

Product Specific Information

PA1-986 detects RFP tagged proteins. This antibody is specific for the RFP tag and does not detect GFP or GFP tagged proteins.

PA1-986 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of RFP tagged proteins. PA1-986 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples.

PA1-986 immunogen is a synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag. This peptide (Cat. # PEP-260) is available for use in neutralization and control experiments.

Target Information

Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.