Thermo Fisher Scientific DYKDDDDK Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 51 publications 51 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 16 publications 16 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 8 publications 8 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 2 publications 2 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: D Y K D D D D K C

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_347227

Product Specific Information

PA1-984B detects the DYKDDDDK epitope tag sequence, similar to the FLAG tag from Sigma.

PA1-984B has been successfully used in Western blot and immunofluorescence procedures to detect the presence of fusion proteins containing the epitope tag sequence DYKDDDDK.

PA1-984B immunizing peptide corresponds to the epitope tag sequence DYKDDDDKC. This peptide (Cat. # PEP-087) is available for use in neutralization and control experiments.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.