Thermo Fisher Scientific BDNF Polyclonal Antibody
Rabbit polyclonal antibody recognizing human BDNF. Validated for WB, IHC, and ICC/IF. Lyophilized form, reconstitutable in sterile water. Suitable for neuroscience research on neuronal survival, differentiation, and synaptic regulation.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the N-terminal region of human mature BDNF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10709853 |
Product Specific Information
- Glycerol (1:1) may be added for additional stability.
- Reconstitute in 500 µL of sterile water.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. It is synthesized as pre-proBDNF, followed by cleavage to proBDNF. The mature 14 kDa protein and pro-BDNF are both biologically active and secreted in synaptic vesicles.
BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several neuron types. Signaling occurs mainly through the TrkB receptor, though p75-NTR binding has also been shown.
BDNF promotes neuronal survival and differentiation, playing a crucial role in memory formation and synaptic regulation. Reduced BDNF expression is observed in Alzheimer’s and Huntington’s diseases and may influence stress response and mood disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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