Thermo Fisher Scientific BDNF Polyclonal Antibody
Rabbit polyclonal antibody targeting mature human BDNF. Validated for WB and IHC applications. Recognizes BDNF across multiple species including mouse and rat. Lyophilized form, reconstitutable in sterile water. Suitable for neuroscience and neurotroph...
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous (PubMed) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the N-terminal region of human mature BDNF conjugated to a blue carrier protein was used as the antigen. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in many species including rat, mouse, zebrafish, and xenopus.
- Specificity: mature BDNF.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) belongs to the neurotrophin family. It is synthesized as pre-proBDNF, then cleaved to proBDNF, and further processed to the mature 14 kDa protein. Both pro-BDNF and mature BDNF are biologically active, secreted in synaptic vesicles.
BDNF is widely expressed in the central nervous system and acts via TrkB and p75-NTR receptors, promoting neuronal survival, differentiation, and synaptic regulation. It plays a vital role in memory formation and neuroplasticity. Reduced expression has been observed in Alzheimer’s and Huntington’s disease and may be involved in stress response and mood disorders.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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