Thermo Fisher Scientific Stat4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

ELISA (ELISA)

1:5,000-1:25,000

-

Immunoprecipitation (IP)

5 µL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 725-749 of Mouse STAT-4. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STAT4,uniProtId:P42228-1,ncbiNodeId:10090,antigenRange:725-749,antigenLength:749,antigenImageFileName:100-401-861_STAT4_P42228-1_Rabbit.svg,antigenImageFileNamePDP:100-401-861_STAT4_P42228-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

85 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reactivity occurs against mouse STAT4 protein. Cross reactivity with closely related STAT family members has not been determined. Cross reactivity is expected against human STAT4 protein based on a 95% homology of the amino acid sequence of the immunogen between human and mouse. A single amino acid change is reported (from N to K). Cross reactivity is expected against rat STAT4.

Target Information

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.