Thermo Fisher Scientific Stat4 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Reactivity occurs against mouse STAT4 protein. Cross reactivity with closely related STAT family members has not been determined. Cross reactivity is expected against human STAT4 protein based on a 95% homology of the amino acid sequence of the immunogen between human and mouse (a single amino acid change from N to K). Cross reactivity is expected against rat STAT4.

Target Information

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity.

STAT4 expression is restricted to the thymus, spleen, and testis. STAT4 is activated by the cytokine interleukin 12 (IL-12), which is required for T-cell independent induction of IFN-gamma—a key step in suppressing bacterial and parasitic infections. IL-12 also supports Th1 response development for defense against intracellular pathogens. STAT4-deficient mice show impaired IL-12 development of Th1 cells and enhanced Th2 cell development. In mouse viral infection, IFN-α/β activation of STAT4 is required for IFN-γ production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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