Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:

• p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
• p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-12. Anti-Human IL-12-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect hIL-12 by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When used with PeproTech Polyclonal Anti-Human IL-12 (500-P154G) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant hIL-12.

Western Blot:
To detect hIL-12 by Western Blot, use 0.1–0.2 µg/mL of this antibody. In conjunction with compatible secondary reagents, the detection limit for recombinant hIL-12 is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.

Package:
500-P154GBT-1MG is provided as 2 × 500 µg.

Target Information

IL-12 p70 (interleukin-12) is a cytokine critical in immune responses to infections and cancer. It is produced by dendritic cells, macrophages, and B cells in response to pathogens and tumor cells. IL-12 p70 stimulates interferon-gamma production and activates T cells and NK cells. It is studied as a therapeutic target for infectious diseases, autoimmune disorders, and cancer due to its immune-enhancing and immunomodulatory roles.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.