Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech
인간 IL-12 p70를 인식하는 염소 유래 Biotin 결합 폴리클로날 항체. Western blot과 ELISA에 적합하며, 항원 친화 크로마토그래피로 정제됨. 면역반응 연구 및 사이토카인 분석용. -20°C 보관, 보존제 무첨가.
- 카탈로그번호
- 500-P154GBT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | 2 publications |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cell-derived Recombinant Human IL-12 p70 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929519 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein
- p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
- p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-12. The anti-Human IL-12-specific antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
For detection of hIL-12 using sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When paired with PeproTech Polyclonal Anti-Human IL-12 (500-P154G) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant hIL-12.
Western Blot:
To detect hIL-12 by Western Blot, use 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant hIL-12 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P154GBT-1MG is provided as 2 × 500 µg.
Target Information
IL-12 p70 (interleukin-12) is a cytokine that plays a critical role in immune responses to infections and cancer. It is produced by immune cells such as dendritic cells, macrophages, and B cells in response to pathogens and tumor cells. IL-12 p70 stimulates interferon-gamma production and promotes activation and proliferation of T cells and NK cells. It has been studied as a therapeutic target for infectious diseases, autoimmune disorders, and cancer due to its ability to enhance immune responses and modulate inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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