Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein

• p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
• p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-12. The anti-Human IL-12-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
For detection of hIL-12 using sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When paired with PeproTech Polyclonal Anti-Human IL-12 (500-P154G) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant hIL-12.

Western Blot:
To detect hIL-12 by Western Blot, use 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant hIL-12 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P154GBT-1MG is provided as 2 × 500 µg.

Target Information

IL-12 p70 (interleukin-12) is a cytokine that plays a critical role in immune responses to infections and cancer. It is produced by immune cells such as dendritic cells, macrophages, and B cells in response to pathogens and tumor cells. IL-12 p70 stimulates interferon-gamma production and promotes activation and proliferation of T cells and NK cells. It has been studied as a therapeutic target for infectious diseases, autoimmune disorders, and cancer due to its ability to enhance immune responses and modulate inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.