Thermo Fisher Scientific Phosphotyrosine Polyclonal Antibody
Rabbit polyclonal anti-phosphotyrosine antibody for detection of phosphorylated tyrosine residues. Suitable for WB, IHC, ELISA, and IP. Affinity purified, unconjugated, liquid form. Ideal for studying signal transduction and oncogenic pathways.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| ELISA | 1:1,000 |
| Immunoprecipitation (IP) | 10.0 µg/500 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 250 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).
Target Information
Tyrosine phosphorylation plays a crucial role in mitogenic signal transduction, cell cycle progression, and oncogenic transformation. Antibodies specific to phosphorylated tyrosine residues are essential for studying tyrosine-phosphorylated proteins and their biochemical pathways. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical assays, especially when secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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