Thermo Fisher Scientific Phosphotyrosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

ELISA (ELISA)

1:1,000

-

Immunoprecipitation (IP)

10.0 µg/500 µg

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Anti-PhosphoTyrosine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

250 µg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store at -20°C prior to opening.

Aliquot contents and freeze at -20°C or below for extended storage.

Centrifuge product if not completely clear after standing at room temperature.

This product is stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.