Thermo Fisher Scientific Phosphothreonine Polyclonal Antibody
Phosphothreonine에 특이적인 Rabbit Polyclonal Antibody로, Western blot, ELISA, IP 등 다양한 응용 가능. Affinity chromatography로 정제된 고순도 항체로, -20°C에서 장기 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.0 µg/mL |
| ELISA | 0.5 µg/mL |
| Immunoprecipitation (IP) | 10.0 µg/mg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PhosphoThreonine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Centrifuge product if not completely clear after standing at room temperature.
- This product is stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).
Target Information
Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine.
There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine.
Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase.
Small amounts of free phosphothreonine can be detected in urine.
Antibodies against phosphothreonine can be used as identifiers in the analysis of peptides, proteins, and enzymes.
[National Center for Biotechnology Information]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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