Thermo Fisher Scientific Phospho-NFkB (Ser276) Polyclonal Antibody
인산화된 NFkB p65 (Ser276)에 특이적인 폴리클로날 항체로, 인간 시료에 반응합니다. Western blot, IHC, ELISA, IP에 사용 가능하며 고순도 친화 크로마토그래피로 정제되었습니다. -20°C에서 안정적으로 보관되며 연구용으로 적합합니다.
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Thermo Fisher Scientific Phospho-NFkB (Ser276) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.0 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This phospho-specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated pS267 is minimal. Cross-reactivity with pS276 phosphorylated p65 from mouse, rat, or other species has not been determined.
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is located on chromosome 11 in humans. Also known as RelA, it belongs to the Rel family of proteins and forms part of the NF-kB complex that heterodimerizes with p50 or p52.
Upon TNFα binding to its receptor, IKK is phosphorylated, leading to phosphorylation and proteasomal degradation of IκB. NF-kB p65 plays a key role in transcriptional regulation of the immunoglobulin κ gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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