Thermo Fisher Scientific CD3e Monoclonal Antibody (FN-18)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 35 publications 35 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 13 publications 13 publications

Product Specifications

Species Reactivity

Non-human primate, Rhesus monkey

Published species

Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FN-18

Immunogen

Rhesus monkey peripheral blood mononuclear cells.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• Request custom conjugation

Form

Lyophilized

Purification

Ion-exchange chromatography

Storage buffer

PBS with 125mM trehalose

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373982

Product Specific Information

Reconstitution: Dissolve the contents of the vial by injection of 0.5 mL sterile distilled water. Lyophilized product is stable for at least one year at 4°C. After reconstitution, the contents can be safely stored at 4°C for one month or for one year at -20°C.

Add 0.02% sodium azide to prevent bacterial growth.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.