Thermo Fisher Scientific Human ROCK1 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 15 amino acids near the amino terminus of human ROCK1.
PEP-1244 can be used as a blocking peptide with polyclonal antibody PA5-21130.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate.
ROCK, one of the effectors of the small GTPase Rho, contributes significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition.
The increase in cellular contractility necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation.
ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain within a pleckstrin-homology region, joined by a variable region containing the Rho-binding domain.
During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its carboxy-terminal inhibitory domain and resulting in deregulated, constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induce phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.