Thermo Fisher Scientific Sheep anti-Human IgG (H+L) Secondary Antibody, HRP
Thermo Fisher의 Sheep anti-Human IgG (H+L) HRP 결합 2차 항체로, Western blot, IHC, ELISA 등에 적합합니다. 인간 IgG 전분자를 면역원으로 사용하며, 높은 특이성과 신호 증폭 효과를 제공합니다. 액상 형태로 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:150,000–1:250,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG whole molecule |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10981836 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies increase versatility by enabling various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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