Thermo Fisher Scientific Sheep anti-Rat IgG (H+L) Secondary Antibody, HRP
Thermo Fisher Scientific의 Sheep anti-Rat IgG (H+L) HRP 결합 2차 항체는 Rat IgG 검출용으로 제작된 고감도 폴리클로날 항체입니다. Western blot, IHC, ELISA 등 다양한 실험에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:60,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG whole molecule |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10979296 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- [AP Conjugate]
- [Biotin Conjugate]
- [FITC Conjugate]
- [TRITC Conjugate]
- [Request Custom Conjugation]
Product Specific Information
PA1-28642 detects Rat IgG from rat samples.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting, or purification of the specified target. Secondary antibodies offer increased versatility, enabling use with many detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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