Thermo Fisher Scientific RAB27A Polyclonal Antibody
Human, Mouse, Rat에서 RAB27A 단백질을 검출하는 Sheep polyclonal 항체. Western blot 및 Immunocytochemistry에 사용 가능. 항원 친화 크로마토그래피로 정제된 동결건조 형태. PBS 및 5% 트레할로스 버퍼에 재구성 가능. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human RAB27A (Ser135–Ala218) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608619 |
Product Specific Information
This antibody detects human, mouse, and rat RAB27A in Western blots and recombinant human RAB27A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human RAB27B is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase-mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
RAB27A plays a role in cytotoxic granule exocytosis in lymphocytes and is required for both granule maturation and granule docking and priming at the immunologic synapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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