Thermo Fisher Scientific Polymyxin B Monoclonal Antibody (45)
Polymyxin B에 특이적으로 결합하는 Mouse IgM 단일클론 항체. Western blot, ELISA, ICC/IF, Flow cytometry 등 다양한 응용 가능. 자유형 및 LPS 결합형 Polymyxin B 모두 인식. Endotoxin 연구 및 LPS 상호작용 분석에 활용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:10 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA | 1:50 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Published Species | Bacteria, Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 45 |
| Immunogen | Polymyxin B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Tissue culture supernatant |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_1086743 |
Product Specific Information
This antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS.
Target Information
Polymyxin B (PMB) is a peptide antibiotic that binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction between PMB and LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region.
PMB is relevant for endotoxin research in two major ways:
- PMB reacts with LPS from many species regardless of serospecificity, allowing its use as a general probe for measuring or detecting LPS or lipid A.
- Binding of PMB to LPS may neutralize the detrimental effects of LPS both in vitro and in vivo.
Antibodies to PMB enable quantitative studies of PMB/LPS interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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