Thermo Fisher Scientific Polymyxin B Monoclonal Antibody (45)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

45

Immunogen

Polymixin B.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1086743

Product Specific Information

This antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS.

Target Information

The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Antibodies to PMB allow for the quantitative study of interaction of PMB/LPS interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.