Thermo Fisher Scientific AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

4.0 µg/mL

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Immunocytochemistry (ICC/IF)

1.25 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

AGO2 11A9

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784637

Product Specific Information

Description: This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), which has been described as a transcriptional regulator by means of RNA interference and endonucleolytic cleavage. The epitope recognized by AGO2 11A9 detects reduced Ago2 by Western Blot and stains Ago2 in the cytoplasm and nucleus in Immunocytochemistry. Ago2 monoclonal antibody (AGO2 11A9) has been validated for the following applications: Western Blot and Immunocytochemistry; AGO2 11A9 does not cross react with murine Ago2.

Applications Reported: This AGO2 11A9 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.

Applications Tested: This AGO2 11A9 antibody has been tested by western blot using lysates from different human cancer cell lines and by immunocytochemistry/immunofluorescence using fixed human U-2 OS cells. This may be used at less than or equal to 4 µg/mL for western blot and at less than or equal to 1.25 µg/mL for immunocytochemistry/immunofluorescence.

Target Information

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or ″Slicer″ activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.