Thermo Fisher Scientific AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience
AGO2 11A9 단클론 항체는 인간 Argonaute 2(Ago2)를 특이적으로 인식하며, Western blot 및 면역세포화학(ICC/IF)에 최적화됨. RNA 간섭 및 유전자 발현 조절 연구에 적합. 쥐 Ago2와 교차반응 없음. 액상 형태로 4°C 보관.
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Thermo Fisher Scientific AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 4.0 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1.25 µg/mL | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AGO2 11A9 |
| Immunogen | Peptide of human AGO2 N-terminus |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784637 |
Product Specific Information
This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), a transcriptional regulator involved in RNA interference and endonucleolytic cleavage.
- Detects reduced Ago2 by Western Blot.
- Stains Ago2 in cytoplasm and nucleus in Immunocytochemistry.
- Validated for Western Blot and Immunocytochemistry/Immunofluorescence.
- Does not cross-react with murine Ago2.
- Tested on human cancer cell lysates and fixed human U-2 OS cells.
- Recommended concentrations: ≤4 µg/mL for WB and ≤1.25 µg/mL for ICC/IF.
Target Information
Ago proteins are broadly expressed in somatic cells and associate with miRNAs, playing key roles in RNA silencing pathways.
Only Ago2 exhibits endonucleolytic “Slicer” activity, enabling miRNA-directed mRNA cleavage when base-pairing is perfect.
In cases of partial complementarity, Ago2 represses translation instead of cleavage.
Ago2 is essential for:
- RNA interference and translational repression
- Heterochromatinization and small RNA-guided gene silencing
- mRNA degradation via RISC complex
- miRNA maturation regulation
Overexpression of Ago2 correlates with tumor growth and survival in cancer patients.
Mouse gene disruption studies show Ago2 is crucial for embryonic, B-lymphoid, and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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