Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (SPM590)

Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (SPM590)

Applications and Tested Dilution

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 activation leads to transcription of genes such as p21waf1, contributing to G1 or G2/M cell cycle arrest.

Structurally, p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for activation and stabilization, while phosphorylation at Ser392 is linked to tumor formation. p53 plays a key role in preventing proliferation of damaged cells and acts as an anticancer agent. It is also associated with aging, oxidative stress, and cognitive function through effects on long-term potentiation (LTP).

In normal cells, p53 levels are low, but in transformed cell lines, expression is elevated and contributes to malignancy. Mutations in the TP53 gene, common in human cancers, disrupt DNA binding and tumor suppressor activity. Germline mutations can also occur in cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.