Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (DO-7)

Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (DO-7)

Applications and Tested Dilution

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest.

Structurally, p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 helps prevent proliferation of damaged cells and acts as a key anticancer factor. It is also implicated in aging and oxidative stress, with elevated levels linked to deficits in long-term potentiation (LTP) in learning and memory.

In normal cells, p53 is expressed at low levels, but in various transformed cell lines, it is highly expressed and contributes to malignancy. Mutations in the TP53 gene disrupt DNA binding and tumor suppressor activity, occurring both as somatic and germline mutations (e.g., Li-Fraumeni syndrome).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.