Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (DO-7)
p53 단백질을 표적하는 DO-7 클론 단일클론 항체로, Western blot 및 면역조직화학(IHC)에 적합합니다. 인간, 소, 비인간 영장류 반응성. 단백질 A/G 정제, PBS buffer, 무보존제. 연구용으로만 사용 가능합니다.
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- 7157-MSM2-P1ABX
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Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (DO-7)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Non-human primate |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DO-7 |
| Immunogen | Recombinant human wild type p53 protein expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest.
Structurally, p53 consists of:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 helps prevent proliferation of damaged cells and acts as a key anticancer factor. It is also implicated in aging and oxidative stress, with elevated levels linked to deficits in long-term potentiation (LTP) in learning and memory.
In normal cells, p53 is expressed at low levels, but in various transformed cell lines, it is highly expressed and contributes to malignancy. Mutations in the TP53 gene disrupt DNA binding and tumor suppressor activity, occurring both as somatic and germline mutations (e.g., Li-Fraumeni syndrome).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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