Thermo Fisher Scientific Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody (ST05-43)
Cytokeratin 10 단백질을 인식하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 일관된 로트 간 품질과 동물 유래 성분이 없는 제형을 제공합니다.
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Thermo Fisher Scientific Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody (ST05-43)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:1,500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST05-43 |
| Immunogen | Synthetic peptide within Human Cytokeratin 10 (aa 541–584) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809471 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Cytokeratins (CK) are intermediate filaments found in epithelial cells, including keratinizing (skin) and non-keratinizing (mesothelial) tissues. Though not typical endothelial markers, some microvascular endothelial cells express cytokeratins.
Over 20 different CKs (40–70 kDa, pI 5–8.5) can be identified via two-dimensional gel electrophoresis. CKs are categorized as:
- Type I: acidic polypeptides
- Type II: basic polypeptides
Each epithelial cell expresses at least one member from each family. Monoclonal antibodies to cytokeratin proteins are valuable for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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