Thermo Fisher Scientific TNFR2 Monoclonal Antibody (22221.311)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: human peripheral blood lymphocytes.
• In neutralization studies, at 0.5–1.0 µg/mL, this antibody blocks 50% of the bioactivity due to 0.3 µg/mL recombinant human sTNF-RII, in the presence of 0.25 ng/mL TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells.
• Anti-Human sTNF-RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF-RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha.
• The ND50 of the antibody is defined as the concentration resulting in a one-half maximal inhibition of soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha and lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha.
The TRAF1/TRAF2 complex recruits apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
TNFR2 mediates most of the metabolic effects of TNF-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.