Thermo Fisher Scientific TNFR2 Monoclonal Antibody (22221.311)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

8-25 µg/mL

-

Flow Cytometry (Flow)

Assay-dependent

-

Neutralization (Neu)

0.5-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

22221.311

Immunogen

Recombinant human sTNF-RII, expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS with 5% trehalose

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_779596

Product Specific Information

Recommended positive controls: human peripheral blood lymphocytes.

In Neutralization studies, at 0.5-1.0 µg/mL, this antibody will block 50% of the bioactivity due to 0.3 µg/mL recombinant human sTNF RII, in the presence of 0.25 ng/mL TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells. Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.