Thermo Fisher Scientific Rb Polyclonal Antibody
Rb 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 727–897 of Human Rb |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.47 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544691 |
Product Specific Information
- Recommended positive controls: Jurkat, A431, CL1-0, CL1-5
- Predicted reactivity: Mouse (96%), Rat (96%), Pig (96%), Chicken (81%), Rhesus Monkey (100%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine residues and forms complexes with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein regulates transcription, and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of the breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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