Thermo Fisher Scientific WIPI2 Polyclonal Antibody
인간 및 랫트에서 반응하는 WIPI2 폴리클로날 항체로, Western blot에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며 PBS 버퍼와 sodium azide를 포함한 액상 형태입니다. 자가포식 관련 단백질 연구에 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 17 amino acid peptide near the amino terminus of human WIPI2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551830 |
Product Specific Information
- A suggested positive control is human testis tissue lysate.
- PA5-34478 can be used with blocking peptide PEP-1520.
Target Information
WD repeat proteins play a role in many essential biological functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions.
WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins.
It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes.
Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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