Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 737, 805
• Brilliant Violet 421, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436, 600, 645, 702, 780)
• Request custom conjugation

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 complexes with CD132 (common gamma chain) to form IL-7R, a type I glycoprotein expressed by immature B cells, thymocytes, and most mature T cells. IL-7 binding activates Jak/STAT signaling pathways essential for lymphocyte development and T-cell homeostasis.

CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs). Cells with low CD127 and high Foxp3 expression are enriched for suppressive Treg populations.
Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (cat. 14-8079).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry at 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Brilliant Violet™ 711 (BV711) emits at 713 nm and is compatible with violet (405 nm) lasers.
Use Brilliant Stain Buffer™ or Super Bright Complete Staining Buffer to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light.
Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution; not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 713 nm; Laser: Violet.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or affiliates, used under license. Powered by Sirigen.

Target Information

CD127 (Interleukin-7 receptor alpha) regulates lymphopoiesis and T/B cell proliferation.
It forms an alpha/gamma heterodimer with IL7R, mediating IL-7 signaling via Jak/STAT pathways.
Dysfunction of CD127 is associated with severe combined immunodeficiency (SCID).

For Research Use Only. Not for use in diagnostic procedures.

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