Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Violet 711

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor™ 700
• APC
• APC-eFluor™ 780
• Biotin
• Brilliant UV 563, 615, 737
• Brilliant Violet 421, 480
• eFluor™ 450
• FITC
• Functional Grade
• NovaFluor™ Blue 510
• PE, PE-eFluor™ 610
• PerCP-eFluor™ 710
• Super Bright 600
• Custom conjugation available

Product Specific Information

The 2E7 monoclonal antibody reacts with mouse CD103 (Integrin αE). CD103 associates with integrin β7 and is expressed on intraepithelial lymphocytes, peripheral lymphocytes, and dendritic epidermal T cells. CD103 binds to E-cadherin and mediates lymphocyte homing to intestinal epithelium.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse lymph node cells, ≤1.0 µg/test
  A test is defined as staining a cell sample in 100 µL volume (10⁵–10⁸ cells/test). Optimize concentration empirically.

Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers with a 405 nm violet laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
BV711 is sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) up to 3 days at 4°C in the dark. BV711 is not compatible with methanol-based fixation.

Excitation/Emission:
407 nm / 713 nm (Violet Laser)

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD103 (ITGAE, Integrin αE) is expressed on mucosa-associated and activated T lymphocytes. It forms heterodimers with β7 integrin and binds E-cadherin, aiding adhesion and activation of intestinal intraepithelial lymphocytes.
Associated diseases include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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