Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), eBioscience
인간 CD3 복합체의 엡실론 서브유닛을 인식하는 OKT3 단클론 항체로, T세포 활성화 및 면역학 연구에 적합합니다. Flow cytometry와 in vitro T세포 활성화 실험에 활용 가능하며, 고순도(>90%)로 정제된 액상 형태 제품입니다.
- 카탈로그번호
- 14-0037-82
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 3 |
| Flow Cytometry (Flow) | 0.25 µg/test | 106 |
| ELISA | - | 6 |
| Immunoprecipitation (IP) | - | 1 |
| Neutralization (Neu) | - | 1 |
| Functional Assay | - | 181 |
| T-Cell Activation (TCA) | - | 5 |
| Affinity Purification (AP) | - | 2 |
| In vitro Assay (IV) | - | 10 |
| Miscellaneous PubMed (Misc) | - | 10 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | TBS, pH 8.0 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_467057 |
Additional Available Formats
- Alexa Fluor 488, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
Description:
The OKT3 monoclonal antibody recognizes an epitope on the epsilon-subunit within the human CD3 complex. It exhibits potent immunosuppressive properties in vivo and has been used effectively in the treatment of renal, heart, and liver allograft rejection. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and expression. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.
Applications Reported:
- Flow cytometric analysis
- In vitro T-cell activation (Functional Grade OKT3 recommended: Product #16-0037-81)
Applications Tested:
- Tested by flow cytometric analysis of normal human peripheral blood cells
- Recommended ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimal titration advised for best performance
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes subunits CD3γ, CD3δ, CD3ε, and CD3ζ, encoded on chromosome 11. These subunits associate with cytoplasmic signal transduction molecules via tyrosine-based motifs. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. Defects in CD3 genes can cause immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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