Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Alexa Fluor 488, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 585
• NovaFluor Yellow 590, 660, 730
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

Description:
The OKT3 monoclonal antibody recognizes an epitope on the epsilon-subunit within the human CD3 complex. It exhibits potent immunosuppressive properties in vivo and has been used effectively in the treatment of renal, heart, and liver allograft rejection. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and expression. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.

Applications Reported:

• Flow cytometric analysis
• In vitro T-cell activation (Functional Grade OKT3 recommended: Product #16-0037-81)

Applications Tested:

• Tested by flow cytometric analysis of normal human peripheral blood cells
• Recommended ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimal titration advised for best performance

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes subunits CD3γ, CD3δ, CD3ε, and CD3ζ, encoded on chromosome 11. These subunits associate with cytoplasmic signal transduction molecules via tyrosine-based motifs. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. Defects in CD3 genes can cause immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.