Thermo Fisher Scientific Osteopontin Polyclonal Antibody, PeproTech
Human Osteopontin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P314-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Osteopontin Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEK293 cells-derived Recombinant Human Osteopontin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930341 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography employing an immobilized Human Osteopontin matrix.
Sandwich ELISA:
To detect Human Osteopontin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Osteopontin (500-P314Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Osteopontin.
Western Blot:
To detect Human Osteopontin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human Osteopontin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding RGD sequence modulates its function and binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a survival advantage and novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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