Thermo Fisher Scientific Glycine Receptor beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyR beta, 2ndintracellular loop if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GLRB,uniProtId:P20781-1,ncbiNodeId:10114,antigenRange:394-405,antigenLength:496,antigenImageFileName:AGR-014-200UL_GLRB_P20781-1_Rabbit.svg,antigenImageFileNamePDP:AGR-014-200UL_GLRB_P20781-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential to voluntary motor control and reflex responses. Glycine binds to glycine receptors (GlyR) in the postsynaptic neuronal membranes. GlyR, gamma-aminobutryic acid, serotonin and acetylcholine comprise an evolutionally conserved superfamily of ligand-gated ion channels. The pentameric subunit structure of GlyR consists of two types of glycosylated membrane proteins, alpha1 through alpha4 and beta, and an associated peripheral membrane protein, which combine to form a chloride-selective ion channel. In humans, the composition of the pentamer changes from alpha2 subunits in the fetal CNS to alpha1 and beta subunits in the adult CNS. Fast potentiation of GlyR by intracellular Ca2+ in the brainstem and midbrain indicate an important role for Ca2+ in modulation of glycinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.