Thermo Fisher Scientific Glycine Receptor beta Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:200
• Publications: -

Product Specifications

Product Specific Information

• Reconstitution:
  Reconstitute with 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
  Ships as a lyophilized powder at room temperature.
  Upon arrival, store at -20°C.
  Reconstituted solution can be stored at 4°C for up to 1 week.
  For longer storage, aliquot and store at -20°C.
  Avoid multiple freeze/thaw cycles.
  Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential for voluntary motor control and reflex responses. Glycine binds to glycine receptors (GlyR) in the postsynaptic neuronal membranes. GlyR, gamma-aminobutyric acid, serotonin, and acetylcholine receptors form an evolutionarily conserved superfamily of ligand-gated ion channels. The pentameric subunit structure of GlyR consists of glycosylated membrane proteins (alpha1–alpha4 and beta) and an associated peripheral membrane protein, forming a chloride-selective ion channel. In humans, the pentamer composition changes from alpha2 subunits in the fetal CNS to alpha1 and beta subunits in the adult CNS. Fast potentiation of GlyR by intracellular Ca²⁺ in the brainstem and midbrain indicates an important role for Ca²⁺ in modulation of glycinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.