Thermo Fisher Scientific beta Lactamase Monoclonal Antibody (8A5.A10)

Applications

Tested Dilution

Publications

Western Blot (WB)

10 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

10-20 µg/mL

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Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8A5.A10

Immunogen

5`-His-tagged E. coli 205 TEM-1 R+ beta-lactamase

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2280996

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Sequence: MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

Target Information

The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.