Thermo Fisher Scientific HDAC1 Monoclonal Antibody (HDAC1-21)
HDAC1 단백질을 인식하는 Mouse IgG3 단일클론 항체로 Western blot, ELISA, IP, ChIP에 사용 가능. 인간 시료에 적합하며 rat에도 교차 반응 예상. 액상 형태로 2.4 mg/mL 농도, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | View 1 publication |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HDAC1-21 |
| Immunogen | Synthetic peptide corresponding to residues K(466) E E K P E A K G V K E E V K L A(482) of human HDAC1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.4 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_559382 |
Product Specific Information
- Expected to cross react with rat due to sequence homology.
- Detects a band of approximately 65 kDa by Western blot.
- Possible positive controls: HeLa cell whole cell lysate.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in regulating eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is part of the histone deacetylase complex. It interacts with the retinoblastoma tumor-suppressor protein, which is crucial for controlling cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effects on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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