Thermo Fisher Scientific BRD7 Recombinant Rabbit Monoclonal Antibody (BLR221K)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Flow Cytometry (Flow)

1 µL/10^6 cells

-

Immunoprecipitation (IP)

12 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR221K

Immunogen

Peptide representing a region between residue 601 and the C-terminus (residue 651) of Human Bromodomain-containing protein 7. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BRD7,uniProtId:Q9NPI1-1,ncbiNodeId:9606,antigenRange:601-651,antigenLength:651,antigenImageFileName:A700-221_BRD7_Q9NPI1-1_Rabbit.svg,antigenImageFileNamePDP:A700-221_BRD7_Q9NPI1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

500 µg/mL

Purification

purified

Storage buffer

BBS, pH 8.2, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE cell sections.

Target Information

BRD7 acts both as coactivator and as corepressor. For example, it activates the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. In addition, it is required for TP53-mediated cell-cycle arrest in response to oncogene activation. BRD7 promotes acetylation of TP53 at Lys-382, and thereby promotes efficient recruitment of TP53 to target promoters, inhibiting cell cycle progression from G1 to S phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.