Thermo Fisher Scientific BRD7 Recombinant Rabbit Monoclonal Antibody (BLR220K)
BRD7 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot과 유세포분석에 최적화됨. 인간 및 생쥐 시료에 반응하며, 고순도 정제 및 안정한 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
Flow Cytometry Note: Cells fixed in 4% formaldehyde and permeabilized with 90% methanol.
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR220K |
| Immunogen | Peptide representing a region between residue 150 and 200 of Human Bromodomain-containing protein 7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
BRD7 acts both as coactivator and as corepressor. It activates the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. In addition, it is required for TP53-mediated cell-cycle arrest in response to oncogene activation. BRD7 promotes acetylation of TP53 at Lys-382, thereby promoting efficient recruitment of TP53 to target promoters and inhibiting cell cycle progression from G1 to S phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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