Thermo Fisher Scientific DKK3 Polyclonal Antibody
Human DKK3 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합. 고순도 항원 친화 크로마토그래피 정제. PBS/glycerol buffer로 안정화되어 장기 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein corresponding to Human DKK3. Recombinant protein control fragment (Product # RP-89882) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2856178 |
Product Specific Information
Immunogen sequence:
CGDQLCVWGH CTKMATRGSN GTICDNQRDC QPGLCCAFQR GLLFPVCTPL PVEGELCHDP ASRLLDLITW ELEPDGALDR CPCASGLLCQ PHSHSLVYVC KPTFVGSRDQ DGEILLPREV PDEYLAASW
Target Information
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a linker region and contain ten cysteine residues each.
The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane.
The linker region contains 50–55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids.
All DKKs have several potential sites for cleavage by furin-type proteases.
Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord.
In situ hybridization reveals highest expression in mouse brain, eye, and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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