Thermo Fisher Scientific Spectrin beta III (SPTBN2) Monoclonal Antibody (SPTBN2, 1583)
Spectrin beta III(SPTBN2)에 특이적인 단일클론 항체로 Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 시료에 반응하며, 비결합형 액상 형태로 제공. Glutamate 수송체 안정화 연구 및 신경퇴행 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPTBN2, 1583 |
| Immunogen | Recombinant fragment (around aa356–475) of human SPTBN2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Spectrins are principal components of the cell membrane-cytoskeleton, composed of two alpha and two beta spectrin subunits.
The SPTBN2 gene encodes spectrin beta non-erythrocytic 2 (beta-III spectrin), which stabilizes the glutamate transporter EAAT4 at the plasma membrane surface.
Mutations in SPTBN2 cause spinocerebellar ataxia type 5 (SCA5), characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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