Thermo Fisher Scientific Spectrin beta III (SPTBN2) Monoclonal Antibody (SPTBN2, 1582)
Spectrin beta III(SPTBN2)에 특이적인 mouse monoclonal antibody로, WB, IHC, ICC, Flow Cytometry 등에 사용 가능. Recombinant fragment(aa356-475)을 면역원으로 제작되었으며, Protein A/G로 정제된 액상 형태. 인간 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPTBN2, 1582 |
| Immunogen | Recombinant fragment (around aa356–475) of human SPTBN2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Spectrins are principal components of the cell membrane–cytoskeleton and are composed of two alpha and two beta spectrin subunits.
The protein encoded by the SPTBN2 gene, also known as spectrin beta non-erythrocytic 2 (βIII-spectrin), is related to but distinct from spectrin beta non-erythrocytic 1 (SPTBN1).
SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the plasma membrane surface.
Mutations in this gene cause spinocerebellar ataxia type 5 (SCA5), characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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