Thermo Fisher Scientific CD218a (IL-18Ra) Monoclonal Antibody (H44), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H44

Conjugate

APC APC APC

View additional formats

• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573258

Product Specific Information

Description: The H44 antibody reacts with the interleukin-18 receptor alpha chain, also known as IL-1Rrp, an IL-1R family member. IL-18R alpha is expressed on Th1 cells, NK cell subset, neutrophils and IL-12-stimulated tonsillar B cells. By itself, IL-18R alpha binds IL-18 with low affinity and when complexed with IL-18R beta chain binds IL-18 with high affinity. The proinflammatory cytokine IL-18 and its receptor complex play an important role in the innate and autoimmune responses.

Applications Reported: This H44 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended to stain whole blood before RBC lysis.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.