Thermo Fisher Scientific IL-21 Monoclonal Antibody (FFA21), APC, eBioscience

Thermo Fisher Scientific IL-21 Monoclonal Antibody (FFA21), APC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 0.125 µg/test
• Publications: View 4 publications

Product Specifications

Available Formats

• eFluor 450
• eFluor 660
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21), a 17 kDa immunomodulatory cytokine mainly produced by NKT, Th17, and TFH cells.
IL-21 signaling via IL-21R and STAT3 promotes transcriptional activation through Bcl6, supporting TFH cell effector function.
It facilitates T cell-dependent B cell differentiation into plasma and memory cells, stimulates IgG production, and induces apoptosis in naive B cells.

In Th17 cells, IL-21 regulates IL-23R expression and balances IRF4, RORγt, and Foxp3 activity. IL-21-deficient mice show resistance to chemically induced colitis due to impaired Th17-associated molecule expression.
Preliminary data indicate that clone FFA21 recognizes a distinct epitope compared to clone mhalx21 (Product # 51-7213-82).

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Th17-polarized splenocytes restimulated with PMA, Ionomycin, and Brefeldin A for 5 hours
• Recommended Usage: ≤0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Excitation: 633–647 nm; Emission: 660 nm; Laser: Red
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-21 is a 17 kDa cytokine produced by NKT, Th17, and TFH cells. It promotes TFH cell development, B cell differentiation, and IgG production, while modulating Th17 maturation through IL-23R and transcriptional regulators STAT3, IRF4, and RORγt.
Deficiency in IL-21 protects mice from chemically induced colitis, whereas elevated IL-21 levels are observed in colitis models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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