Thermo Fisher Scientific Ion AmpliSeq Comprehensive Cancer Panel

Ion AmpliSeq™ Comprehensive Cancer Panel

The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Covering over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is one of the most comprehensive cancer gene panels available. With all-exon coverage of 409 genes, it delivers fast, FFPE-compatible target selection for a broad survey of key genes for semiconductor sequencing.

View the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (PDF)

Key Features and Benefits

• Broad survey of 409 key genes in a single PCR reaction, no additional equipment required
• 16,000 primer pairs in four pools using Ion AmpliSeq™ technology
• Low DNA input (40 ng) enables use with FFPE and needle biopsy samples
• Pre-designed primer pools for rapid start of genomic studies
• Simplified variant analysis with Ion Reporter™ software

Comprehensive Gene Coverage

Developed in collaboration with leading cancer researchers, this panel targets all exons of tumor suppressor genes and oncogenes most frequently cited and mutated in cancer. It enables simultaneous analysis of coding sequences and splice variants across multiple gene families, including pathways related to cancer drivers, drug targets, apoptosis, DNA repair, transcription regulation, inflammation, and growth factors.
All genes from the Ion AmpliSeq™ Cancer Panel are included, providing complementary data within a single assay.

Unmatched Multiplex PCR Performance

Comprising four primer pools with nearly 16,000 primer pairs covering 409 genes, the panel achieves high uniformity and specificity when used with the Ion AmpliSeq™ Library Kit 2.0, advancing multiplex PCR technology.

Low DNA Input Requirement

Requires only 40 ng of DNA, enabling analysis of limited or degraded samples such as FFPE and fine needle aspiration biopsies. Short amplicon design (average 155 bp) ensures compatibility with challenging sample types.

Simplified Target Selection

Pre-designed primers eliminate the need for gene or primer design, allowing immediate generation of results. Researchers can further customize panels using Ion AmpliSeq™ Designer to add or remove genes as needed.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

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