Thermo Fisher Scientific HSF1 Polyclonal Antibody
Rabbit polyclonal antibody against human HSF1 protein. Validated for WB and ELISA with high specificity. Affinity purified and supplied as liquid form at 1 mg/mL. Recommended storage at -20°C. For research use only.
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- 600-401-GV3x (2개 옵션)
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:50,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified Anti-HSF1 antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminal portion of human HSF1 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-HSF1 is directed against human HSF1 at an N-terminus position. A BLAST analysis suggests reactivity with this protein in mouse and bovine species based on 100% homology for the immunogen sequence.
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and present upstream of all heat shock genes.
The HSEs are highly conserved among species, yet HSF purified from yeast, Drosophila, and human have different molecular weights and do not show significant immunological cross-reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli: HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA binding activity. Upon stress, HSF1 becomes activated to a nuclear localized, trimeric state that binds DNA. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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